The Imaging Facility provides the infrastructure to support state-of-the-art microscopy at the FLI. Currently, the Facility operates and maintains several widefield and confocal microscopes, a Lightsheet and a STED superresolution microscope.

The facility staff provides introduction into microscopy techniques and service for FLI researchers and, upon arrangement, also for external users.

The Imaging Facility provides the infrastructure to support state-of-the-art microscopy at the FLI. Currently, the Facility operates and maintains several widefield, confocal, lightsheet (STED-) superresolution, microdissection, optical tweezer and multi-slide scanning  microscopes.

The facility staff provides introduction into microscopy techniques and service for FLI researchers and, on a collaborative basis, also for external users.

The Facility is specialized in live cell microscopy to monitor molecule and cellular dynamics in space and time. Several fluorescence fluctuation microscopy (FFM) techniques with single-molecule resolution have been established including FRAP, FCS, FCCS, FLIM, FRET, and RICS.

Equipment and techniques available:

• LSM/ConfoCor3 (FRET, FRAP, FCS, FCCS, RICS and time-lapse)
• Airyscan (multi-fluorescence, rapid live-cell imaging, superresolution)
• PALM Microdissection/Optical Tweezer
• Apotome optical sectioning (Widefield, brightfield,quasi-confocal fluorescence microscopy; colocalization analysis, 2D, 3D, distance measurements, tiling of tissue sections)
• Multi-Slide Scanners (up to 100
• Several Axiovert 200 ApoTomes; 2x inverse, 3x upright (optical sectioning with structured illumination)
• TCS SP8 STED-3X/FALCON (Stimulated emission depletion super-resolution microscopy; FLIM)
• Lightsheet Z1 (live imaging of whole organisms in 3D over time).
• Image quantitation (Huygens, Imrais, Arivis, Metamorph)